GRIN Lenses & Streamlined Workflow Solutions
Workflow Simplified with Inscopix Solution
At Inscopix, we offer state-of-the-art miniscope platforms that allow for high-resolution imaging of calcium dynamics in freely behaving animals. Our technology incorporates genetically encoded calcium indicators and Gradient Refractive Index (GRIN) lenses to enable precise imaging of deep brain regions.
Our surgical workflow solutions expedite your research, enabling maximum cell capture and faster imaging results.
Our end-to-end solution includes:
Ready-to-Image (RTI) Virus
The only commercially available diluted GCaMP virus that is functionally validated for all Inscopix miniscope applications.
- GCaMP viruses optimized for several brain regions.
- Ensures consistent and reproducible GCaMP expression.
- Available in ready-to-inject aliquots for immediate use.
Gradient Index (GRIN) Lenses
GRIN lenses use a continuous change in refractive index within the lens material, replacing complex curved surfaces with flat optical surfaces. Light rays continuously bend within the lens until they converge at a focal point.
Inscopix lenses are specialized GRIN lenses designed for in-vivo imaging, addressing diverse neuroscience research needs in non-human subjects. Our range of lens options is optimized for calcium imaging applications. Explore our lens options below and contact us for more information.
ProView™ Integrated Lenses
Our ProView Integrated lenses and ProView DC Integrated lenses have a miniscope-compatible baseplate with an attached GRIN lens. When combined with our RTI viruses, they streamline surgical procedures and produce optimal imaging results. The ProView DC Integrated Lenses are optimized for dual-color imaging to minimize chromatic aberration.
- Facilitate efficient surgeries and ensure consistent, reproducible imaging across cohorts
- Optimize the gap between the objective and GRIN lens for a consistent working distance range
- Provide both straight and prism choices for versatile applications
ProView Lenses
ProView lenses are GRIN lenses with an attached cuff. They are designed to be used with the ProView Implant Kit for secure holding and easier insertion of the lens during implantation.
- Improve surgical efficiency and time while reducing complications during implantation
- Protective cuff shield the lens and ensures optical durability
- Offer both straight and prism choices for versatile applications
GRIN Lenses
GRIN lenses used for single-photon (1P) excitation microscopy.
- Straight lenses: Designed for imaging cell layers within a working distance of ~0-200 μm beneath the lens implantation site
- Prism lenses: Offered a 90-degree side-view imaging capability, avoiding direct implantation over cells of interest and capturing multi-layered views within a working distance of ~0-300 μm.
2P & ProView 2P GRIN Lenses
Two-photon (2P) GRIN Lenses and the ProView 2P GRIN Lenses with customized cuff are expertly designed for optimal 2P excitation microscopy.
Inscopix offers a range of 2P GRIN lenses that support customer applications for 2P imaging including multiphoton microscopes.
- Capture detailed images deep within biological tissues with minimal damage
- Improve surgical efficiency and time while reducing complications during implantation with ProView 2P
- Doublet 2P GRIN lens option offers 2.5x magnification enhancing cellular resolution
ProView Implant Kit
ProView Implant Kit is specifically designed to secure the ProView lens and ProView 2P GRIN lens.
- Compatible with most stereotax
- Supports precise implantation coordinates
- Increases surgical yield and minimizes damage
Multi-region GRIN Lenses
GRIN lenses used for the Bruker multi-region miniscope imaging system.
GRIN lens with connectors:
- Designed for imaging cell layers within a working distance of ~0-250 μm beneath the lens implantation site
- Connector attached to GRIN lens ensures reproducible imaging success
- Customizable lengths available upon request
Applications Kits
Achieve immediate imaging results with our fully packaged solution for calcium imaging, which includes reagents, lenses, and validated protocols for brain-region specific research.
- Easy-to-use kits for specific brain regions
- Optimized reagents, lenses and protocols for faster imaging success
- Quality-controlled and validated in-vivo to ensure consistent and reproducible imaging data
Additional Resources
Learn how Inscopix is simplifying the surgical workflow for your neural circuit imaging.
Watch our Lunch ‘n Learn webinar on optimizing your miniscope imaging workflow.
Discover what multiphoton microscopy and a 2P GRIN lens can reveal deep in the brain.