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Calcium imaging of adult-born neurons in freely moving mice

Authors: Alvaro Carrier-Ruiz, Yuki Sugaya, Deependra Kumar, Pablo Vergara, Iyo Koyanagi, Sakthivel Srinivasan, Toshie Naoi, Masanobu Kano, Masanori Sakaguchi
Publication: STAR Protocols
Date: March 19, 2021
Link to article: https://www.sciencedirect.com/science/article/pii/S2666166720302252?via%3Dihub=


Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca2+ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips.

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