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Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics


Authors: D. A. Doronin, N. V. Barykina, O. M. Subach, V. P. Sotskov, V. V. Plusnin, O. A. Ivleva, E. A. Isaakova, A. M. Varizhuk, G. E. Pozmogova, A. Y. Malyshev, I. V. Smirnov, K. D. Piatkevich, K. V. Anokhin, G. N. Enikolopov, F. V. Subach
Publication: BMC Biotechnology
Date: February 13, 2018
Link to article: https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-018-0417-2


We demonstrate that expanding the family of NTnC-like calcium indicators is a promising strategy for the development of the next generation of GECIs with smaller molecule size and lower Ca2+ ions buffering capacity as compared with commonly used GECIs.

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