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Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins

Skyler L. Jackman et al.
Cell Reports | March 20, 2018
Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically...

Efficient and accurate extraction of in vivo calcium signals from microendoscopic video data

Pengcheng Zhou et al.
eLife | February 22, 2018
In vivo calcium imaging through microendoscopic lenses enables imaging of previously inaccessible neuronal populations deep within the brains of freely moving animals. However, it is computationally challenging to...

Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

D. A. Doronin et al.
BMC Biotechnology | February 13, 2018
We demonstrate that expanding the family of NTnC-like calcium indicators is a promising strategy for the development of the next generation of GECIs with smaller molecule size and lower Ca2+ ions buffering capacity as...

Skin suturing and cortical surface viral infusion improves imaging of neuronal ensemble activity with head-mounted miniature microscopes

Xinjian Li et al.
Journal of Neuroscience Methods | August 19, 2017
In vivo optical imaging of neural activity provides important insights into brain functions at the single-cell level. Cranial windows and virally delivered calcium indicators are commonly used for imaging cortical...

In vivo imaging of neural activity

Weijian Yang et al.
Nature Methods | March 31, 2017
Since the introduction of calcium imaging to monitor neuronal activity with single-cell resolution, optical imaging methods have revolutionized neuroscience by enabling systematic recordings of neuronal circuits in...

A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites

Natalia V. Barykina et al.
Scientific Reports | September 28, 2016
Genetically encoded calcium indicators (GECIs) are mainly represented by two- or one-fluorophore-based sensors. One type of two-fluorophore-based sensor, carrying Opsanus troponin C (TnC) as the Ca2+-binding moiety, has...

Calcium imaging of basal forebrain activity during innate and learned behavio

Thomas C. Harrison et al.
Frontiers in Neural Circuits | May 9, 2016
The basal forebrain (BF) plays crucial roles in arousal, attention, and memory, and its impairment is associated with a variety of cognitive deficits. The BF consists of cholinergic, GABAergic, and glutamatergic...

Large-scale fluorescence calcium-imaging methods for studies of long-term memory in behaving mammals

Pablo Jercog et al.
Cold Spring Harbor Perspectives In Biology | April 5, 2016
During long-term memory formation, cellular and molecular processes reshape how individual neurons respond to specific patterns of synaptic input. It remains poorly understood how such changes impact information...

Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses

Shanna L Resendez et al.
Nature Protocols | February 25, 2016
Genetically encoded calcium indicators for visualizing dynamic cellular activity have greatly expanded our understanding of the brain. However, owing to the light-scattering properties of the brain, as well as the size...

Miniature microscopes for large-scale imaging of neuronal activity in freely behaving rodents

Yaniv Ziv et al.
Current Opinion in Neurobiology | May 16, 2015
Recording neuronal activity in behaving subjects has been instrumental in studying how information is represented and processed by the brain. Recent advances in optical imaging and bioengineering have converged to...

Cellular level brain imaging in behaving mammals: an engineering approach

Elizabeth J. O. Hamel et al.
Neuron | April 8, 2015
Fluorescence imaging offers expanding capabilities for recording neural dynamics in behaving mammals, including the means to monitor hundreds of cells targeted by genetic type or connectivity, track cells over weeks,...

Tools for resolving functional activity and connectivity within intact neural circuits

Joshua H. Jennings et al.
Current Biology | January 6, 2014
Mammalian neural circuits are sophisticated biological systems that choreograph behavioral processes vital for survival. While the inherent complexity of discrete neural circuits has proven difficult to decipher, many...

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